FLOW CYTOMETRY



  1. INTRODUCTION
  2. HISTORY
  3. DEFINITIONS
  4. TECHNICAL PRINCIPLES OF FLOW CYTOMETRY
    1. CELL SAMPLES
    2. HYDRODYNAMIC FOCUSING
    3. CIRCUITS OPTIQUES
    4. COLLECTION OF EMITTED LIGHT
    5. SIGNALS COLLECTED
      1. Scattered light
      2. Threshold
      3. Emitted fluorescence
    6. SIGNAL CONVERSION
    7. PRESENTATION OF RESULTS
  5. CELL SORTING
  6. SPECTRAL CYTOMETRY
  7. AVANTAGES AND LIMITATIONS OF FLOW CYTOMETRY
    1. QUANTITATIVE ANALYSIS
    2. DETECTION SENSITIVITY
    3. WORKING SPEED
    4. SIMULTANEOUS ANALYSIS OF MULTIPLE PARAMETERS
    5. CELL SORTING
  8. MAJOR APPLICATIONS
    1. IMMUNOFLUORESCENCE FOR CELL CARACTERISATION
      1. Principles of immunofluorescence
      2. Principles of fluorescence compensation
      3. Determination of the positivity threshold
    2. SPECIFIC DNA LABELLING
      1. Specific DNA labelling
      2. Stoichiometry of staining
      3. Doublets removing
      4. Cell viability
      5. DNA quantitation
      6. Cell cycle
    3. APOPTOSIS MEASUREMENT
      1. DNA fragmentation measurement
      2. Annexine V labelling
      3. Multiparamétric analysis
    4. OTHER APPLICATIONS
      1. Reporter genes
      2. Protein quantitation
      3. Cell proliferation
      4. Stem cell analysis


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