Canto 2
Flow
cytometry analysis of 2 physical parameters and 8 fluorescences.
This station is controlled by the Diva 8.1 software (Becton
Dickinson).
Laser 488nm
Filter 1 (530/40): FITC, EGFP, CFSE, TO-PRO-1, Alexa 488
Filter 2 (585/42): PE, SNARF, DyeCycle Orange, Alexa 546, DiOC
Filter 3 (670 LP): PE-Cy5.5, PerCP-Cy5.5, Tri-Color, 7AAD, Alexa 700
Filter 4 (780/60): PE-Cy7, Alexa 750
Laser 638 nm
Filter 5 (660/20): APC, Alexa647, Cy5, TO-PRO-3, SYTOX Red
Filter 6 (780/60P): APC-Cy7, APC-Alexa 750, APC-H7
Laser 405 nm
Filter 7 (450/50): Pacific Blue, Alexa 405, PO-PRO-1, DyeCycle
Violet
Filter 8 (510/50) :AmCyan, SYTOX Blue, BD horizon V500, Pacific
orange,
Use
- Cellular analysis of 10 parameters including 8 fluorescences.
- Intracellular or surface labeling, fixed or live.
- Ultra-fast acquisition up to 10 000 events per second.
N.B. :
Minimum quantity of samples to be provided: Volume 300
microliters, Number of cells 50,000
Material with radioactive labeling not accepted