Flow
cytometry analysis of 2 physical parameters and 10 fluorescences.
This station is controlled by the Kaluza Aquisition software
(Beckman Coulter).
Laser 488nm, 22 mW
Filter 1 (525/40): FITC, EGFP, CFSE, TO-PRO-1, Alexa 488
Filter 2 (575/30): PE, SNARF, DyeCycle Orange, Alexa 546, DiOC
Filter 3 (620/30): PE-Texas Red, PI, DiOC
Filter 4 (695/30): PE-Cy5.5, PerCP-Cy5.5, Tri-Color, 7AAD, Alexa 700
Filter 5 (755LP): PE-Cy7, Alexa 750
Laser 638 nm, 25mW
Filter 6 (660/20): APC, Alexa647, Cy5, TO-PRO-3, SYTOX Red
Filter 7 (725/20): Alexa 700
Filter 8 (755LP): APC-Cy7, APC-Alexa 750, APC-H7
Laser 405 nm, 40mW
Filter 9 (450/40): Pacific Blue, Alexa 405, PO-PRO-1, DyeCycle
Violet, SYTOX Blue
Filter 10 (550/40): AmCyan,
Use
- Cellular analysis of 12 parameters including 10 fluorescences
- Intracellular or surface labeling, fixed or live
- Large number of events (up to 24,000,000 events per file depending
on the number of parameters saved)
- Acquisition of up to 25 000 events per second.
N.B. :
Minimum quantity of samples to be provided: Volume 300
microliters, Number of cells 50,000
Material with radioactive labeling not accepted
