Not all fluorochromes have the same performance, so you will not detect the same antigen with the same antibody coupled to different fluorochromes.

Here is an example found in a supplier's catalog:

Rendement Fluorochromes

Therefore you should choose your fluorochrome carefully. The lowest represented antigens should be revealed with antibodies coupled to fluorochromes that have the best yield, keeping in mind that some instruments cannot measure certain fluorochromes.

Remember that the yield decreases in this order for these fluorochromes:

APC > PE > PE-Cy5 > PerCP-Cy5.5 >> FITC >> PerCP

To help you to choose, here are several links :

A free open-source site with spectra of a multitude of fluorochromes and in particular fluorescent proteins : https://www.fpbase.org/spectra/

Commercial sites on fluorochromes :

Cytek BDBiosciences Thermo Fischer Biolegend Chroma


A tool to design your panels :  Chromocyte
Finally an interesting document from Invitrogen : Download (pdf format)