Location

1 Aurora CS, 4 lasers (407 nm, 488 nm, 561 nm,  638 nm), 30 fluorescences, cloning unit, PSM.   1 Aurora Analyzer, 4 lasers (407 nm, 488 nm, 561 nm,  638 nm), PSM.     1 Gallios (3 lasers 488, 638, 405 nm, 10 fluorescences, sample changer)    1 Canto II (3 lasers 488, 638, 405 nm, 8 fluorescences, sample changer)

Several data processing stations with the following software : FlowJo, Diva, Kaluza, SpectroFlow.

1 clean room in L2 containment for cell sorting.

It is requested, for each publication that required the use of the device, that the department be listed in the acknowledgements.

Objective :

The initial objective was to open a high-performance flow cytometry center, able to provide multiparametric analysis and sorting. Integrated in the IRMB and being part of MRI, the laboratory participates in the work of regional laboratories. This laboratory, which does not have its own theme, is widely open to the regional scientific community: INSERM, CNRS, University of Sciences, Faculty of Pharmacy, IFREMER, IRD, CIRAD, INRA, ENSAM... In recent years, the technical evolution of the equipment has led many laboratories to analyze and purify cells by means of flow cytometry to better clarify their role or analyze their content (protein, DNA, RNA... ), whether human, animal, plant, bacterial, marine... The needs of laboratories are increasing with the evolution of technologies, many studies require the use of analyzers and high performance sorters.

Activity :

For more details on these themes, go  Here.

The laboratory has participated in the testing of various devices from the 2 largest cytometer manufacturers, Becton Dickinson and Beckman Coulter.