FLOW CYTOMETRY
- INTRODUCTION
- HISTORY
-
DEFINITIONS
-
TECHNICAL PRINCIPLES OF FLOW CYTOMETRY
- CELL SAMPLES
- HYDRODYNAMIC FOCUSING
- CIRCUITS OPTIQUES
- COLLECTION OF EMITTED LIGHT
- SIGNALS COLLECTED
- Scattered light
- Threshold
- Emitted fluorescence
- SIGNAL CONVERSION
- PRESENTATION OF RESULTS
- CELL
SORTING
- SPECTRAL
CYTOMETRY
- AVANTAGES
AND LIMITATIONS OF FLOW CYTOMETRY
- QUANTITATIVE ANALYSIS
- DETECTION SENSITIVITY
- WORKING SPEED
- SIMULTANEOUS ANALYSIS OF MULTIPLE PARAMETERS
- CELL SORTING
- MAJOR APPLICATIONS
- IMMUNOFLUORESCENCE
FOR CELL CARACTERISATION
- Principles of immunofluorescence
- Principles of fluorescence compensation
- Determination of the positivity threshold
- SPECIFIC DNA
LABELLING
- Specific DNA labelling
- Stoichiometry of staining
- Doublets removing
- Cell viability
- DNA quantitation
- Cell cycle
- APOPTOSIS
MEASUREMENT
- DNA fragmentation measurement
- Annexine V labelling
- Multiparamétric analysis
- OTHER
APPLICATIONS
- Reporter genes
- Protein quantitation
- Cell proliferation
- Stem cell analysis